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Title: The effect of soil salinity and pepino mosaic virus infection on the expression of tomato defence-related genes
Authors: Zarrougui, Nour Elhouda
Spanos, Theodore
Kissoudis, Christos
Livieratos, Ioannis C.
Subjects LC: Genomics
Plants - Effects of stress on
Plant diseases
Keywords: Abiotic stress
Biotic stress
Pepino Mosaic Virus (PepMV)
Plant defence
Solanum lycopersicum
Issue Date: 7-Apr-2022
Publisher: J. Wiley & Sons
Abstract: To reflect field conditions where cultivated plants are frequently exposed to multiple stresses simultaneously, the effect of salinity on the expression of defence-related genes in tomato plants was examined in the absence and presence of pepino mosaic virus (PepMV) infection. Tomato seedlings were grown under three soil salinity regimes from the fourth day after transplantation: (a) no-added salt (control), (b) low salinity (20 mM NaCl) and (c) high salinity (100 mM NaCl). The plants were non-, mock- or PepMV-inoculated 14 days after transplantation and systemic leaves were collected 4 days postinoculation for analysis by reverse transcription-quantitative PCR (RT-qPCR). In noninoculated tomato plants exposed to low and high salinity, expression of the salicylic acid (SA)-, jasmonic acid-, ethylene-related genes was typically down-regulated compared to control plants, with only a slight (nonsignificant) increase in ABA2 expression. In PepMV-infected tomato plants, a strong induction of SA-related genes was observed, which was compromised under low and high salt stress. The induction of PR1 gene expression by PepMV infection was the highest (an 82-fold increase) in control plants, but was significantly less (a 20-fold increase) under conditions of low and high soil salinity. No direct correlation between SA gene expression levels and PepMV RNA accumulation became apparent. In RT-qPCR, the levels of PepMV genomic RNA and tomato LoxD and ACS1 RNA transcripts under mild salt stress were significantly reduced (the former 6-fold), but under high salt stress the levels of all were similar to those in the nonstressed controls.
Description: This journal is edited by the British Society for Plant Pathology.
Type: Journal Article
Relation (Part Of): International Journal of Plant Pathology
Publication Status: Published
Citation: Zarrougui, NE, Spanos, T, & Kissoudis, C 2022, 'The effect of soil salinity and pepino mosaic virus infection on the expression of tomato defence-related genes', Plant Pathology, vol. 71, no.1.
Restrictions: All rights reserved
Attribution-NonCommercial-NoDerivatives 4.0 International
Language: en
Appears in Collections:Journal Articles

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