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Title: | Optimisation of the HS-SPME/GC-MS approach by design of experiments combined with chemometrics for the classification of Cretan virgin olive oils |
Authors: | Lioupi, Artemis Sampsonidis, Ioannis Virgiliou, Christina Papoti, Vassiliki T. Zinoviadou, Kyriaki Spyros, Apostolos Theodoridis, Georgios |
Editors: | Torta, Federico Tesio |
Subjects LC: | Olive oil Olive oil - Analysis |
Keywords: | Gas chromatography-mass spectrometry (GC-MS) Headspace-solid phase microextraction (HS-SPME) Olive oil Volatile organic compounds (VOCs) Volatilomics Foodomics Metabolomics |
Issue Date: | 25-Jan-2022 |
Publisher: | MDPI |
Abstract: | A headspace-solid phase microextraction/gas chromatography-mass spectrometry (HS-SPME/GC-MS) method was developed herein for the analysis of virgin olive oil volatile metabolome. Optimisation of SPME conditions was performed by Design of Experiments (DoE) and Response Surface Methodology (RSM) approaches and factors, such as sample volume, sample stirring, extraction temperature and time, and desorption temperature and time, were examined to reach optimal microextraction conditions. The potential of the optimised method was then investigated for its use in the classification of Cretan virgin olive oil samples with the aid of multivariate statistical analysis. Certain markers were identified with significance in the geographical classification of Cretan extra-virgin olive oil (EVOO) samples. In total, 92 volatile organic compounds were tentatively identified and semi-quantified, and the data obtained confirm that the method is robust, reliable, and analytically powerful for olive oil classification. |
Length: | 19 pages |
Type: | Journal Article |
Relation (Part Of): | Metabolites Journal |
Publication Status: | Published |
URI: | https://www.mdpi.com/2218-1989/12/2/114/htm https://doi.org/10.3390/metabo12020114 http://repository.afs.edu.gr/handle/6000/449 |
Citation: | Lioupi, A, Sampsonidis, I, Virgiliou, C, Papoti, VT, Zinoviadou, KG, Spyros, A, & Theodoridis, G 2022, "Optimisation of the HS-SPME/GC-MS approach by design of experiments combined with chemometrics for the classification of Cretan virgin olive oils", Metabolites, vol. 12, no.114, pp. 1-19. |
Repository: | DAPL |
Restrictions: | Open Access Attribution 4.0 International |
Language: | en |
Appears in Collections: | Journal Articles |
Files in This Item:
File | Description | Size | Format | |
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metabolites-12-00114-v4.pdf | 2.37 MB | Adobe PDF | View/Open |
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