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Τίτλος: Comprehensive approaches reveal key transcripts and metabolites highlighting metabolic diversity among three oriental tobacco varieties
Συγγραφείς: Tsaballa, Aphrodite
Sarrou, Eirini
Xanthopoulou, Aliki
Tsaliki, Eleni
Kissoudis, Christos
Karagiannis, Evangelos
Michailidis, Michail
Martens, Stefan
Sperdouli, Elektra
Hilioti, Zoe
Fotopoulos, Vasileios
Nianiou-Obeidat, Irini
Tsaftaris, Athanasios
Madesis, Panagiotis
Kalivas, Apostolos
Ganopoulos, Ioannis
Θέματα LC: Genomics
RNA
Tobacco - Varieties
Λέξεις-Κλειδιά: N. tabacum
RNA-seq
Leaf transcriptomics
GC-MS
Metabolomics
Ημερομηνία Έκδοσης: 2020
Εκδότης: Elsevier
Επιτομή: Tobacco is an economically important crop with leaves that are a significant source of aromatic, bioactive compounds such as phenolics and alkaloids. In the present study un-biased transcriptomics and metabolomics approaches were employed to identify and quantify individual changes in transcript and metabolite profiles in leaves of three oriental tobacco varieties. Based on next generation sequencing (NGS) and gas chromatography mass spectrometry (GC–MS) technologies, a wide variety of transcripts and metabolites was detected and the metabolic diversity among varieties was determined. Genes with largest expression differences were identified in the leaves of the three varieties; among them three were commonly over-expressed in two varieties in comparison with the third variety. Notably, significant expression differences were recorded in phenylalanine ammonia lyase (PAL) genes that are key genes of phenylpropanoid biosynthesis. Following transcriptomics, metabolomics analysis has shown that polyphenolic compounds varied widely among the three varieties. Furthermore, statistically significant differences in soluble sugars, alcohols, organic acids, amino acids and other metabolites were also revealed. The integration of the two -omics datasets in determining diversity of tobacco varieties offers important readouts for the genetic control of metabolite production and constitutes a resource for future studies in the area of plant biotechnology for improving tobacco specific traits.
Έκταση: 11 pages
Τύπος: Journal Article
Συσχέτιση (Μέρος του): Industrial Crops & Products journal
Κατάσταση Δημοσίευσης: Published
URI: https://doi.org/10.1016/j.indcrop.2019.111933
http://repository.afs.edu.gr/handle/6000/352
ISSN: 0926-6690
Αναφορά: Tsaballa, A, Sarrou, E, Xanthopoulou, A, Tsaliki, E, Kissoudis, C, Karagiannis, E, Michailidis, M, Martens, S, Sperdouli, E, Hilioti, Z, Fotopoulos, V, Nianiou-Obeidat, I, Tsaftaris, A, Madesis, P, Kalivas, A, & Ganopoulos, I 2020, 'Comprehensive approaches reveal key transcripts and metabolites highlighting metabolic diversity among three oriental tobacco varieties', Industrial Crops & Products, vol. 143, pp. 1-11.
Δικαιώματα: All rights reserved
Attribution-NonCommercial-NoDerivatives 4.0 International
Γλώσσα: en_US
Εμφανίζεται στις Συλλογές: Άρθρα σε επιστημονικά περιοδικά

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